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We collected the black precipitate using centrifugation and allowed it to dry overnight at room temperature
Br Med Bull 1997, 53:307-321
Subsequently, E3 ligases either behave as bona fide enzymes (HECT E3s), or a matchmaker (RING E3s), to transfer ubiquitin from a charged E2 to substrates, facilitating the formation of an isopeptide bond between the C terminal glycine of ubiquitin and substrate lysine residue 1 (Figure 1)
Wnt and hedgehog signaling pathways in bone development
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